SpectraMax® iD5

Reference:

The SpectraMax® iD5 Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection.

Read modes Absorbance Fluorescence (top/bottom read)
Luminescence (top/bottom read)
Time-Resolved Fluorescence (top/bottom read)
Fluorescence Polarization (top/bottom read)
HTRF (top/bottom read)
HTRF
Wavelength ranges Abs: 230 - 1000 nm
FL Ex: 250 - 830 nm
FL Em: 270 - 850 nm
Lumi: 300 - 850 nm
TRF Ex: 350 nm
TRF Em: 450 - 750 nm
FP: 300 - 750 nm
Wavelength selection Hybrid filters and monochromator tunable in 1 nm increments
Absorbance photometric accuracy/linearity < ±0.003 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV
Absorbance photometric precision/repeatability < ±0.003 OD ±1.0%, 0 - 3 OD VIS 0 - 3 OD UV
Sensitivity top read optimized FL: Mono: 1 pM fluorescein 96 well, 1 pM fluorescein 384 well; Filters: 0.3 pM fluoresein 96 well, 0.5 pM fluorescein 384 well Lumi: 2 pM ATP 96 well, 4 pM ATP 384 well TRF (filters with enhanced TRF module): 10 fM Europium (2 amol/well) 96 well, 10 fM Europium (1 amol/well) 384 well FP: Mono: 2 mP @ 10nM 96 well, 2 mP @ 10nM 384 well; Filters: 1 mP @ 1 nM 96 well, 2 mP @ 1 nM 384 well
Light source(s) High power xenon flash lamp
Detector(s) Silicon photodiode
Ultra-cooled photomultiplier tube
Read types (endpoint, kinetic, etc) Endpoint
Kinetic
Spectrum scan
Well scan
Read times (kinetic interval for 96-well plate)* Abs: 28 seconds
FL: 21 seconds
Lumi: 21 seconds
TRF: 25 seconds
FP: 35 seconds
Plate shaking Linear
Orbital
Double orbital
Injectors Yes
Western Blot Yes
Robotics / automation Yes
Plate type(s) 6 - 384 well plates
Temperature control Ambient + 5°C to 66°C
Dimensions H 40.1 cm x W 53.2 cm x D 59.8 cm
Validation tools

Absorbance validation plate
Fluorescence validation plate
Luminescence validation plate
Multi-mode validation plate
SoftMax® Pro GxP Software

For more information visit Molecular Devices website

Specific References